中国科学院深圳先进技术研究院机构知识库(SIAT OpenIR): MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP
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MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP
Liang WC; Wang Y; Liang PP; Pan XQ; Fu WM; Yeung VS; Lu YF; Wan DC; Tsui SK; Tsang SY; Ma WB; Zhang JF; Waye M
2015
Source PublicationJOURNAL OF CELLULAR BIOCHEMISTRY
Subtype期刊论文
AbstractIn the past decade, miRNA emerges as a vital player in orchestrating gene regulation and maintaining cellular homeostasis. It is well documented that miRNA influences a variety of biological events, including embryogenesis, cell fate decision, and cellular differentiation. Adipogenesis is an organized process of cellular differentiation by which pre-adipocytes differentiate towards mature adipocytes. It has been shown that adipogenesis is tightly modulated by a number of transcription factors such as PPARγ, KLF4, and C/EBPα. However, the molecular mechanisms underlying the missing link between miRNA and adipogenesis-related transcription factors remain elusive. In this study, we unveiled that miR-25, a member of miR-106b-25 cluster, was remarkably downregulated during 3T3-L1 adipogenesis. Restored expression of miR-25 significantly impaired 3T3-L1 adipogenesis and downregulated the expression of serial adipogenesis-related genes. Further experiments presented that ectopic expression of miR-25 did not affect cell proliferation and cell cycle progression. Finally, KLF4 and C/EBPα, two key regulators of adipocyte differentiation, were experimentally identified as bona fide targets for miR-25. These data indicate that miR-25 is a novel negative regulator of adipocyte differentiation and it suppressed 3T3-L1 adipogenesis by targeting KLF4 and C/EBPα, which provides novel insights into the molecular mechanism of miRNA-mediated cellular differentiation.
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Indexed BySCI
Language英语
Department南沙所-生物
Document Type期刊论文
Identifierhttp://ir.siat.ac.cn/handle/172644/7554
Collection南沙所
AffiliationJOURNAL OF CELLULAR BIOCHEMISTRY
Recommended Citation
GB/T 7714
Liang WC,Wang Y,Liang PP,et al. MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2015.
APA Liang WC.,Wang Y.,Liang PP.,Pan XQ.,Fu WM.,...&Waye M.(2015).MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP.JOURNAL OF CELLULAR BIOCHEMISTRY.
MLA Liang WC,et al."MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP".JOURNAL OF CELLULAR BIOCHEMISTRY (2015).
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