中国科学院深圳先进技术研究院机构知识库(SIAT OpenIR): Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing
SIAT OpenIR  > 集成所
Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing
Zixun Wang; Yang Yang; Zhen Xu; Ying Wang; Wenjun Zhang; Peng Shi
2015
Source PublicationNANO LETTERS
Subtype期刊论文
AbstractUnderstanding intracellular signaling cascades and network is one of the core topics in modern biology. Novel tools based on nanotechnologies have enabled probing and analyzing intracellular signaling with unprecedented sensitivity and specificity. In this study, we developed a minimally invasive method for in situ probing specific signaling components of cellular innate immunity in living cells. The technique was based on diamond-nanoneedlearrays functionalized with aptamer-based molecular sensors, which were inserted into cytoplasmic domain using a centrifugation controlled process to capture molecular targets. Simultaneously, these diamond-nanoneedles also facilitated the delivery of double-strand DNAs (dsDNA90) into cells to activate the pathway involving the stimulator of interferon genes (STING). We showed that the nanoneedle-based biosensors can be successfully utilized to isolate transcriptional factor, NF-kappa B, from intracellular regions without damaging the cells, upon STING activation. By using a reversible protocol and repeated probing in living cells, we were able to examine the singling dynamics of NF-kappa B, which was quickly translocated from cytoplasm to nucleus region within similar to 40 min of intracellular introduction of dsDNA90 for both A549 and neuron cells. These results demonstrated a novel and versatile tool for targeted in situ dissection of intracellular signaling, providing the potential to resolve new sights into various cellular processes.
URL查看原文
Indexed BySCI
Language英语
Department功能薄膜材料研究中心
Document Type期刊论文
Identifierhttp://ir.siat.ac.cn/handle/172644/6485
Collection集成所
AffiliationNANO LETTERS
Recommended Citation
GB/T 7714
Zixun Wang,Yang Yang,Zhen Xu,et al. Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing[J]. NANO LETTERS,2015.
APA Zixun Wang,Yang Yang,Zhen Xu,Ying Wang,Wenjun Zhang,&Peng Shi.(2015).Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing.NANO LETTERS.
MLA Zixun Wang,et al."Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing".NANO LETTERS (2015).
Files in This Item: Download All
File Name/Size DocType Version Access License
功能薄膜2015008.pdf(6204KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zixun Wang]'s Articles
[Yang Yang]'s Articles
[Zhen Xu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zixun Wang]'s Articles
[Yang Yang]'s Articles
[Zhen Xu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zixun Wang]'s Articles
[Yang Yang]'s Articles
[Zhen Xu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 功能薄膜2015008.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.