中国科学院深圳先进技术研究院机构知识库(SIAT OpenIR): Structural modularity of the XIST ribonucleoprotein complex
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Structural modularity of the XIST ribonucleoprotein complex
Zhipeng Lu; Jimmy K. Guo; Yuning Wei; Diana R. Dou; Brian Zarnegar; Qing Ma; Rui Li; Yang Zhao; Fan Liu; Hani Choudhry; Paul A. Khavari; Howard Y. Chang
2020
Source PublicationNature Communications
Subtype期刊论文
AbstractLong noncoding RNAs are thought to regulate gene expression by organizing protein complexes through unclear mechanisms. XIST controls the inactivation of an entire X chromosome in female placental mammals. Here we develop and integrate several orthogonal structure-interaction methods to demonstrate that XIST RNA-protein complex folds into an evolutionarily conserved modular architecture. Chimeric RNAs and clustered protein binding in fRIP and eCLIP experiments align with long-range RNA secondary structure, revealing discrete XIST domains that interact with distinct sets of effector proteins. CRISPR-Cas9-mediated permutation of the Xist A-repeat location shows that A-repeat serves as a nucleation center for multiple Xist-associated proteins and m6A modification. Thus modular architecture plays an essential role, in addition to sequence motifs, in determining the specificity of RBP binding and m6A modification. Together, this work builds a comprehensive structure-function model for the XIST RNA-protein complex, and suggests a general strategy for mechanistic studies of large ribonucleoprotein assemblies.
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Indexed BySCI
Language英语
EI Accession Number合成基因组学研究中心
Document Type期刊论文
Identifierhttp://ir.siat.ac.cn/handle/172644/16936
Collection合成所
Recommended Citation
GB/T 7714
Zhipeng Lu,Jimmy K. Guo,Yuning Wei,et al. Structural modularity of the XIST ribonucleoprotein complex[J]. Nature Communications,2020.
APA Zhipeng Lu.,Jimmy K. Guo.,Yuning Wei.,Diana R. Dou.,Brian Zarnegar.,...&Howard Y. Chang.(2020).Structural modularity of the XIST ribonucleoprotein complex.Nature Communications.
MLA Zhipeng Lu,et al."Structural modularity of the XIST ribonucleoprotein complex".Nature Communications (2020).
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