中国科学院深圳先进技术研究院机构知识库(SIAT OpenIR): Droplet facilitated microfluidicsinglecell processor for transcriptome sequencing
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Droplet facilitated microfluidicsinglecell processor for transcriptome sequencing
B. Y. Zhang; X. Hong; W. L. Shu; H. T. Feng; J. J. Wang; J. Zhong; Y. Chen
2016
Conference NameInternational Conference of Microfluidics, Nanofluidics and Lab-on-a-Chip
Conference PlaceDalian
AbstractSingle cell whole transcriptome analysis is a powerful approach for understanding changes in gene expression within an isogenic cell population, and microfluidic device represents a useful tool for high-throughput single-cell analysis. Multilayer microfluidic devices with integrated valves provide an ideal platform for single cell manipulation and analysis, and they have been used extensively in single cell genetic analysis. However, the reaction process of library construction of single cell sequencing is complicated, and using conventional bench top method to do pre-sequencing process is still a common way for many laboratories. Therefore, we aim to develop a useful microfluidic platform to perform single cell extraction with simple operation and high efficiency, and conveniently convert them to bench top experiments.
Department医工所微纳系统与仿生医学研究中心
Indexed By其他
Language英语
Document Type会议论文
Identifierhttp://ir.siat.ac.cn/handle/172644/10597
Collection医工所
Affiliation2016
Recommended Citation
GB/T 7714
B. Y. Zhang,X. Hong,W. L. Shu,et al. Droplet facilitated microfluidicsinglecell processor for transcriptome sequencing[C],2016.
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