中国科学院深圳先进技术研究院机构知识库(SIAT OpenIR): Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
SIAT OpenIR  > 医工所
Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
Zheng, Li-Zhen; Wang, Xin-Luan; Cao, Hui-Juan; Chen, Shi-Hui; Huang, Le; Qin, Ling
2016
Source PublicationBONE
Subtype期刊论文
AbstractIn an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroidmethylprednisolone (MPS). At 2, 4, and 6 weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively. Two fluorescent dyes xylenol orange and calcein green were injected before sacrificing the animals for in vivo labeling of the newly formed bone. At week 6 after induction, proximal femora of rabbits were dissected for micro-CT and histological analysis. Results showed significant bone lossin the metaphysis of femoral head in the control rabbits after SAON induction. Src siRNA treatment was able to prevent steroid-associate bone loss intrabecular bone and increase cortical bone thickness at femoral neck. Histomorphometry showed that Src siRNA increased the osteoblastic bone formation and decreased the eroded bone surfaces suggesting decreased osteoclastic bone resorption. This was the first study to report bone loss after SAON induction in rabbit model that could be prevented by knocking down Src by siRNA.
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Indexed BySCI
Language英语
Department转化医学
Document Type期刊论文
Identifierhttp://ir.siat.ac.cn/handle/172644/10521
Collection医工所
AffiliationBONE
Recommended Citation
GB/T 7714
Zheng, Li-Zhen,Wang, Xin-Luan,Cao, Hui-Juan,et al. Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model[J]. BONE,2016.
APA Zheng, Li-Zhen,Wang, Xin-Luan,Cao, Hui-Juan,Chen, Shi-Hui,Huang, Le,&Qin, Ling.(2016).Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model.BONE.
MLA Zheng, Li-Zhen,et al."Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model".BONE (2016).
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